The application period for our Partnership Project Program is now closed. Announcements of the awardees are expected late summer 2018. Thank you for your submissions!

News

McMahon Lab publishes JASN papers on human kidney development

Feb 15, 2018

Dr. Andy McMahon, director of the Stem Cell Institute, USC, and his lab published three papers in the Journal of the American Society of Nephrology (JASN) today that detail several similarities and differences in how the kidney develops in mice and humans - at the molecular, cellular and genetic levels. This massive amount of data will be crucial in helping researchers with the ultimate goal of building kidneys from human cells while working from mouse blueprints and adds a human component for kidney research that has never before been available.

Nephron

At an early stage, a nephron forming in the human kidney generates an S-shaped structure. Green cells will generate the kidneys’ filtering device, and blue and red cells specialized regions responsible for distinct nephron activities. Image courtesy of Stacy Moroz and Tracy Tran/Andy McMahon Lab, USC Stem Cell

The paper citations are as follow:

Lindström NO, McMahon J, Guo J, Tran T, Guo Q, Rutledge E, Parvez RK, Saribekyan G, Schuler RE, Liao C, Kim AD, Abdelhalim A, Ruffins SW, Thornton ME, Basking L, Grubbs B, Kesselman C, McMahon AP. Conserved and Divergent Features of Human and Mouse Kidney Organogenesis. JASN. 2018 Feb 15. doi: 10.1681/ASN.2017080887

Lindström NO, Guo J, Kim AD, Tran T, Guo Q, Brandine GS, Ransick A, Parvez RK, Thornton ME, Basking L, Grubbs B, McMahon JA, Smith AD, McMahon AP. Conserved and Divergent Features of Mesenchymal Progenitor Cell Types within the Cortical Nephrogenic Niche of the Human and Mouse Kidney. JASN. 2018 Feb 15. doi: 10.1681/ASN.2017080890

Lindström NO, Tran T, Guo J, Rutledge E, Parvez RK, Thornton ME, Grubbs B, McMahon JA, McMahon AP. Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning. JASN. 2018 Feb 15. doi: 10.1681/ASN.2017091036

You can find all referenced images and data organized into “collections” (much like a virtual “album”) here.

These collections were made using the underlying DERIVA software that is powering the new GUDMAP data browser (which launches officially in April). Read more about collections here.

RBK Partnership Pilot Program Open for 2018

Feb 5, 2018

The (Re)Building A Kidney Consortium is happy to announce that we are ready to accept new submissions to collaborate with us via our Partnership Program.

Specifically, we are looking for additional projects in the following subject areas:

Current subject areas of interest are LIMITED to Physiologic Function and Repair/Regeneration:

1. Physiologic Function

It is critical that bioengineered devices and biologicals (e.g. organoids, bioprinted devices) developed in RBK reflect the physiologic functions of the kidney. Specialized tools and expertise are needed to help evaluate these functions. Successful applications must involve a collaboration with an existing RBK member (https://www.rebuildingakidney.org/projects) and propose to evaluate physiological function in RBK developed bioengineered devices or biologicals. Examples might include:

  • Develop novel way to non-invasively image renal function. Engineer or apply physiologically-relevant biosensors and reporters to image/measure physiologic processes in real-time.
  • Perfuse organoids or bioengineered devices and assess flow-dependent function (e.g. PAH secretion; glucose, Na+ and HCO3- reabsorption in the proximal tubule; K+ and H+ secretion in distal tubule).
  • Implement classic physiological methods of polarization, resistance, filtration, reabsorption and secretion.

2. Repair/Regeneration

It remains a major focus of the RBK to better understand productive repair in response to injury. Applicants may propose either a new standalone project OR a collaboration with an existing RBK member (https://www.rebuildingakidney.org/projects). Examples might include:

  • Profile endogenous repair/regeneration pathways in cells and tissues. When appropriate, the use of human samples is strongly encouraged.
  • Define repair circuits to identify drug targets that will enhance endogenous repair and develop target-based small screening assays. Use phenotype screening assays to identify small molecules that will activate innate reparative responses.
  • Discover and/or characterize signals released by injured tissue required for productive repair and regeneration.
  • Application of cells, including induced pluripotent stem (iPS) cells, and regenerative therapies to repair and reverse injury. Develop novel ways to preferentially target cells to the kidney and to non-invasively image the integration and/or "fusion" of applied stem cells. Develop methods for identifying, counting and eliminating potentially harmful components and cells from stem cell preparations.
  • Develop novel ways to non-invasively image repair and regeneration, perhaps by imaging the integration and/or "fusion" of applied stem cells.

For complete details about this program, go to:

rebuildingakidney.org/partnership-project-program.html

Several Funding Opportunities in Somatic Cell Genome Editing

Feb 2, 2018

The Genome Editing Research Program has posted several funding opportunities:

https://commonfund.nih.gov/editing/fundingopportunities

If interested, please note that the LOIs are due now and the applications are due in early April.