Bioengineering

• 3D Bioprinting of Vascularized, Convoluted Renal Proximal Tubules (Jennifer Lewis, Harvard University)

• Application of Progenitor Niche Signals to Ex Vivo Nephrogenesis (Leif Oxburgh, Maine Medical Center / Thomas Carroll, University of Texas Southwestern Medical Center / Ondine Cleaver, University of Texas Southwestern Medical Center / David Kaplan, Tufts University)

• Building Extracellular Matrix Scaffolds to Induce Nephron Development (Jason A. Wertheim, Northwestern University and Jesse Brown VA Medical Center)

• Cytokines and Extracellular Matrix (Jeffrey A. Hubbell, University of Chicago)

• Rebuilding the Glomerular Filtration Barrier by Regenerating Adult Podocytes (Stuart J. Shankland, University of Washington / Ying Zheng, University of Washington)

Cell-type specific transcriptional profiling

• Cellular Diversity in Human Nephrogenesis (Andrew McMahon, University of Southern California)

• Transcriptional Profiling of In Vivo Derived Human Kidney Tissue Using Single Cell RNA Sequencing (Jason Spence, University of Michigan)

Coordinating Center

• (Re)Building the Kidney Coordinating Center (Carl Kesselman, University of Southern California)

Endogenous Repair

• Adult Progenitors in Kidney Tubulointerstitium (Benjamin D. Humphreys, Washington University in St. Louis)

Generation of complex kidney structures

• Generating an Interconnected Kidney Tubule Architecture (Iain A. Drummond, Massachusetts General Hospital)

Growth and differentiation

• Differentiation of Human Pluripotent Stem Cells into Kidney Cell Lineages (Oliver Wessely, Cleveland Clinic / Jan Jensen, Cleveland Clinic)

Organoids

• Developing Pro-regenerative Drug Therapies for Acute Kidney Injury (Neil Hukriede, University of Pittsburgh / Alan Davidson, University of Auckland)

• Generating Kidney Organoids and Mature Renal Cell Types Via Directed iPSC Differentiation (Melissa H. Little, Murdoch Childrens Research Institute)

Protocol - Analysis of renal biopsies

• Human Kidney Biopsy Single Cell Protocols and Analysis (Benjamin D. Humphreys, Washington University in St. Louis)

• Identifying Kidney Cell Phenotype Factors Using Single Cell RNA Sequencing (Junhyong Kim, University of Pennsylvania / James Eberwine, University of Pennsylvania)

• Using Cold Active Proteases for Single Cell Dissociation (S. Steven Potter, Cincinnati Children's Hospital)

Protocol - Proteomic profiling

• Spatially-preserved Expression Analysis of Kidney Cells in Human Biopsy Tissue (Lloyd Cantley, Yale University)

iPSC reporter lines

• Differentiation of Fluorescent Reporter Human iPS Cell Lines for Monitoring Renal Cell Lineages (Todd Valerius, Brigham and Women's Hospital)

• Engineering Human Collecting System Reporter Pluripotent Stem Cell Lines (Sanjay Jain, Washington University in St. Louis)