Bioengineering

• 3D Bioprinting of Vascularized, Convoluted Renal Proximal Tubules
  (Jennifer Lewis, Harvard University)

• Application of Progenitor Niche Signals to Ex Vivo Nephrogenesis
  (Leif Oxburgh, Maine Medical Center / Thomas Carroll, University of Texas Southwestern Medical Center / Ondine Cleaver, University of Texas Southwestern Medical Center / David Kaplan, Tufts University)

• Building Extracellular Matrix Scaffolds to Induce Nephron Development
  (Jason A. Wertheim, Northwestern University and Jesse Brown VA Medical Center)

• Cytokines and Extracellular Matrix
  (Jeffrey A. Hubbell, University of Chicago)

• Rebuilding the Glomerular Filtration Barrier by Regenerating Adult Podocytes
  (Stuart J. Shankland, University of Washington / Ying Zheng, University of Washington)

• Kidney Microphysiological Analysis Platforms (MAP) to Optimize Function and Model Disease
  (Joseph Bonventre, Brigham and Women's Hospital / Luke Lee, Brigham and Women's Hospital)

Cell-type specific transcriptional profiling

• Cellular Diversity in Human Nephrogenesis
  (Andrew McMahon, University of Southern California)

• Identifying Mechanisms of Renal Repair
  (Andrew McMahon, University of Southern California)

• Transcriptional Profiling of In Vivo Derived Human Kidney Tissue Using Single Cell RNA Sequencing
  (Jason Spence, University of Michigan)

Coordinating Center

• ReBuilding the Kidney Coordinating Center
  (Carl Kesselman, University of Southern California)

Endogenous Repair

• Adult Progenitors in Kidney Tubulointerstitium
  (Benjamin D. Humphreys, Washington University in St. Louis)

Generation of complex kidney structures

• Generating an Interconnected Kidney Tubule Architecture
  (Iain A. Drummond, Massachusetts General Hospital)

Growth and differentiation

• Differentiation of Human Pluripotent Stem Cells into Kidney Cell Lineages
  (Oliver Wessely, Cleveland Clinic / Jan Jensen, Cleveland Clinic)

Organoids

• Developing Pro-regenerative Drug Therapies for Acute Kidney Injury
  (Neil Hukriede, University of Pittsburgh / Alan Davidson, University of Auckland)

• Generating Kidney Organoids and Mature Renal Cell Types Via Directed iPSC Differentiation
  (Melissa H. Little, Murdoch Childrens Research Institute)

Physiology

• Mechanoregulation of Cation Transport in Bioprinted Collecting Ducts
  (Lisa Satlin, Icahn School of Medicine at Mount Sinai)

• Physiologic Properties of Human Kidney Organoids
  (Thomas Kleyman, University of Pittsburgh)

Protocol - Analysis of renal biopsies

• Human Kidney Biopsy Single Cell Protocols and Analysis
  (Benjamin D. Humphreys, Washington University in St. Louis)

• Identifying Kidney Cell Phenotype Factors Using Single Cell RNA Sequencing
  (Junhyong Kim, University of Pennsylvania / James Eberwine, University of Pennsylvania)

• Using Cold Active Proteases for Single Cell Dissociation
  (S. Steven Potter, Cincinnati Children's Hospital)

Protocol - Proteomic profiling

• Spatially-preserved Expression Analysis of Kidney Cells in Human Biopsy Tissue
  (Lloyd Cantley, Yale University)

iPSC reporter lines

• Differentiation of Fluorescent Reporter Human iPS Cell Lines for Monitoring Renal Cell Lineages
  (Todd Valerius, Brigham and Women's Hospital)

• Engineering Human Collecting System Reporter Pluripotent Stem Cell Lines
  (Sanjay Jain, Washington University in St. Louis)